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Cells developed steatosis after incubation with free fatty acids (FFA) (sodium oleate,botanical slimming soft gels mzt, sodium palmitate 2:1) (0.25 mM 1 mM) for 24 hours. 0.5 mM FFA was the highest concentration of FFA which rendered HepG2 cells steatotic without causing apoptosis. Steatotic HepG2 cells were sensitised to apoptosis initiated by tumour necrosis factor alpha (TNF but not Fas ligand (CH11), hydrogen peroxide (H2O2), ultraviolet (UV) irradiation or Camptothecin (CMP), suggesting that the mechanism by which steatosis sensitised HepG2 cells to apoptosis was not a global effect, but specific to TNF death receptor pathways.

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