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Here we show that 21 nucleotide siRNA duplexes specifically suppress expression of endogenous and heterologous genes in different mammalian cell lines, including human embryonic kidney (293) and HeLa cells. Therefore, 21 nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene specific therapeutics.Uptake of dsRNA by insect cell lines has previously been shown to ‘knock down’ the expression of specific proteins, owing to sequence specific, dsRNA mediated mRNA degradation6, 10, 11, 12. son efectivas las pastillas fruta planta The manufacturers of treadmills recommend that a treadmill belt be oiled on the underside of the belt. To do this, loosen the belt and spray the lubricant on the decking where you walk. Then tighten the belt and walk for five minutes to evenly distribute the lubricant. In order to do the same with essential oils, do not dilute the oil. Instead pour a small amount, equivalent to a spray of silicone lubricant on the decking and spread it evenly. Then tighten the belt and walk on it just as you would if you had used the silicone spray.
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