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Luciferase expression from pGL2 is approximately 10 fold lower than from pGL3 in transfected mammalian cells. The region targeted by the siRNA duplexes is indicated as black bar below the coding region of the luciferase genes. B, The sense (top) and antisense (bottom) sequences of the siRNA duplexes targeting GL2, GL3, and RL luciferase are shown. ! 7 day slimming pill The neighbors claim that the high traffic volume and noise from the concerts cause problems for them for weeks surrounding these big events. Since Garth Brooks’ five shows would definitely bring a huge turnout and require a lot of planning and organizing, the residents did have a point. The Dublin City Council sided with the Croke Park Residents Association and refused to grant Garth Brooks a license for more than three nights..
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