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Cells were incubated 20 h after transfection and appeared healthy thereafter. Luciferase expression was subsequently monitored with the Dual luciferase assay (Promega). Transfection efficiencies were determined by fluorescence microscopy for mammalian cells lines after co transfection of 1.1 g hGFP encoding pAD3 (ref. 21) and 0.21 g inverted GL2 siRNA, and were 70 Reporter plasmids were amplified in XL 1 Blue (Stratagene) and purified using the Qiagen EndoFree Maxi Plasmid Kit.Transfection of siRNAs for targeting endogenous genes was carried out using Oligofectamine (Life Technologies) and 0.84 g siRNA duplex per well, but it was recently found that as little as 0.01 g siRNAs per well are sufficient to mediate silencing. chinese bpollen Esolyt+ friends friendsMODERATOR OFwhat’s this?TROPHY CASEGoogle makes an operating system called Android and gives it to companies. Samsung, HTC, etc. can ship phones with it. But they also gave everybody the right to change Android so these companies don just ship the original Android, but they customize it, remove some things, add some things, add carrier apps, emphasize their own brand, change the design. When they ship this modified version of Android, it called a custom ROM. But Android fans who know about the original Android usually don love these changes. So they made their own custom ROM called Cyanogenmod. But this is a different kind of custom ROM. Instead of Samsung or any other big company, it made by just a bunch of passionate guys. And instead of these big companies, it not trying to emphasize any brand or modify the core design of Android. It just original Android + some cool extra features.